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Granuloerythropoietic colonies in human bone marrow, peripheral blood, and
cord blood
AA Fauser and HA Messner
Colonies that contain granulopoietic and erythropoietic cells can be grown
in specimens of human bone marrow, peripheral blood, and cord blood. Growth
of these colonies is promoted by media conditioned by leukocytes in the
presence of phytohemagglutinin (PHA-LCM) and the addition of erythropoietin
on days 4 or 5 to the cultures. Sedimentation velocity profiles for these
granuloerythrocytic colonies suggest their origin from single cells
(CFU-G/E) rather than from doublets or clumps. This hypothesis is supported
by cocultivation of male and female specimens. Cells in granuloerythrocytic
colonies that developed in such mixing experiments were either uniformly
female by Y- chromatin analysis or contained Y-chromatin bodies in both the
granulocytic and erythroid cells.
Volume 52,
Issue 6,
pp. 1243-1248,
12/01/1978
Copyright © 1978 by The American Society of Hematology
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