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MG Farace, E Ullu, A Fantoni, GB Rossi, L Cioe and A Dolei
The poly (A)-containing nuclear RNA from dimethylsulfoxide-induced Friend
leukemia cells was fractionated by acrylamide gel electrophoresis in
denaturing conditions and analyzed for alpha and beta globin RNA sequences.
The results indicate that nuclear RNA contains one species of large-size
RNA (0.6 X 10(6) daltons), which is the putative precursor for beta globin
mRNA only. In addition, it was shown by electrophoretic analysis that the
complex of RNA molecules not resolved by sucrose gradient centrifugation
(11S) comprises sequences of decreasing size (0.34, 0.28, and 0.26 X 10(6)
daltons), which might be the precursors of alpha and beta globin mRNA.
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