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Identification of megakaryocytes, macrophages, and eosinophils in colonies
of human bone marrow containing neurtophilic granulocytes and erythroblasts
AA Fauser and HA Messner
Pluripotent hemopoietic progenitors in human bone marrow can be identified
by their ability to give rise in culture to colonies that contain more than
one lineage of hemopoietic differentiation. Growth of these mixed colonies
is supported by media conditioned by leukocytes in the presence of
phytohemagglutinin (PHA-LCM) and erythropoietin. They can be readily
recognized by direct microscopic observation because of their composition
of colorless cells and cells with a red appearance typical for hemoglobin.
Seventy-three individual mixed colonies from 6 individuals were prepared by
cytocentrifugation for further cytological examination. In addition to
neutrophilic granulocytes and erythroblasts, megakaryocytes were present in
16 of the 73 colonies, as identified by positive reaction for acid
phosphatase. Macrophages were found in 24 and eosinophils in 6 of the 73
colonies.
Volume 53,
Issue 5,
pp. 1023-1027,
05/01/1979
Copyright © 1979 by The American Society of Hematology
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