SEARCH:   Advanced

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Stern, M. Articles by Rosse, W. F. Search for Related Content PubMed PubMed Citation Articles by Stern, M. Articles by Rosse, W. F. Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Two populations of granulocytes in paroxysmal nocturnal hemoglobinuria M Stern and WF Rosse The granulocytes in paroxysmal nocturnal hemoglobinuria (PNH) are defective, and the defect is similar to that previously described for the PNH erythrocyte. Using anti-I antibody to activate complement and 51Cr release to detect cell lysis, we found two populations of granulocytes that differed in their susceptibility to lysis by complement in 5 of 6 patients. A proportion of the cells were lysed by one-fifteenth to one-twentieth the amount of complement required to lyse normal cells; the remainder of the granulocytes appeared to be normal in their susceptibility to the lytic action of complement. The binding of the third component of complement (C3) to PNH granulocytes was at least twice that bound to normal cells, even though the binding of antibody was the same for normal and PNH cells. This suggests that the binding of C3 and probably the efficiency of the terminal steps of complement lysis are increased in the abnormal PHN granulocyte. These defects affect only a portion of the granulocytes, thus suggesting that the disorder is a clonal stem cell abnormality. Volume 53, Issue 5, pp. 928-934, 05/01/1979 Copyright © 1979 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: T. Shichishima, Y. Saitoh, T. Terasawa, K. Ogawa, and Y. Maruyama Relationship Between the Phenotypes of Circulating Erythrocytes and Cultured Erythroblasts in Paroxysmal Nocturnal Hemoglobinuria Blood, July 1, 1997; 90(1): 435 - 443. [Abstract] [Full Text] [PDF]

	Copyright © 1979 by American Society of Hematology         Online ISSN: 1528-0020