Preferential inhibition of murine macrophage colony formation by
prostaglandin E
N Williams
Murine bone marrow progenitor cells that gave rise to macrophage colonies
in semisolid agar were found to be more sensitive to prostaglandins of the
E series (PGE) than were precursor cells of granulocytes and
megakaryocytes. Macrophage colonies themselves had different sensitivities
to the molecule. Precursor cells of macrophages that formed colonies in the
presence of a stimulating activity from L cells (L-cell CSA) were inhibited
to 50% levels by 3 x 10(-9)-M PGE. Macrophage progenitor cells, which
require both L-cell CSA and rat hemolysate for colony growth, were
inhibited to the same level by 3 x 10(-7)-M PGE. Other colony types
(granulocytes and megakaryocytes) were sensitive to PGE only at
concentrations greater than 10(-6) M. Accordingly, addition of different
PGE concentrations to the culture assay should allow easy detection of
precursor cells with morphologically distinct end cells. The different
sensitivities to PGE of two macrophage colony types of different maturation
stages indicate that PGE may provide feedback to control macrophage
formation by inhibiting proliferation and differentiation of immature
monocytoid cells.
Volume 53,
Issue 6,
pp. 1089-1094,
06/01/1979
Copyright © 1979 by The American Society of Hematology
