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Effects of oxygen tension and pH on the respiratory burst of human
neutrophils
TG Gabig, SI Bearman and BM Babior
The respiratory burst of human neutrophils was measured under conditions of
hypoxia and low pH. O2 -- production by neutrophils activated with
opsonized zymosan fell slowly as the oxygen concentration declined to 1%,
then dropped more sharply, reaching negligible levels at oxygen
concentrations less than 0.25%. Production was half maximal at an oxygen
concentration of 0.35% (equivalent to approximately 10-microM dissolved
oxygen). O2- production by the cell- free O2- -forming system prepared from
zymosan-activated neutrophils showed a similar dependence on oxygen
concentration. A drop in pH caused decreases in both oxygen consumption and
O2-- production by zymosan-treated neutrophils, values at PH 6.0 being
10%--20% of those observed at pH 7.5. Experiments with the cell-free O2--
-forming system suggested that this decline in respiratory burst activity
at low pH was due to inefficient activation of the O2-- -forming enzyme
under acidic conditions.
Volume 53,
Issue 6,
pp. 1133-1139,
06/01/1979
Copyright © 1979 by The American Society of Hematology
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