Fractionation of antibodies to L-cell colony-stimulating factor by affinity
chromatography
RK Shadduck, A Waheed, G Pigoli, F Boegel and L Higgins
Purified L-cell colony-stimulating factor (CSF) was coupled to cyanogen-
bromide-activated Sepharose and used to selectively fractionate antibodies
to this factor. With the use of a simplified two-step washing and elution
technique, there was 50%--70% binding of the anti- CSF, with recovery of
60%--100% of the bound material. Both the native antiserum and purified
anti-CSF fractions were inhibitory to murine granulocyte-macrophage colony
formation. The purified antibodies contained only IgG and were reduced in
protein concentration to 0.1% of the serum IgG values. These fractions
should prove useful tools for the study of granulocyte and macrophage
differentiation.
Volume 53,
Issue 6,
pp. 1182-1190,
06/01/1979
Copyright © 1979 by The American Society of Hematology
