Lymphocyte-mediated lysis of antibody coated human red cells in the
presence of human serum
RJ Kurlander and WF Rosse
When peripheral blood lymphocytes and human red cells coated with IgG were
incubated in vitro in culture medium, antibody-dependent
lymphocyte-mediated lysis was observed. This lysis was markedly inhibited
by the addition of purified monoclonal IgG1 (1000 microgram/ml) to the
culture medium. In contrast, lysis by lymphocytes of sensitized red cells
in the presence of undiluted human serum was equal to or greater than lysis
in medium alone, even in the presence of IgG1 at 1000 microgram/ml, despite
the high concentration of IgG in human serum (6000--19,000 microgram/ml).
Serum heated to 56 degrees C for 30 min also restored lysis in the presence
of IgG1. When serum was separated into three fractions by passage through a
Sephadex G-200 column, the third fraction, which contained proteins with a
molecular weight of less than 100,000 d (but neither of the other two
fractions nor purified human albumin), restored lymphocyte-mediated lysis
in the presence of IgG1.
Volume 53,
Issue 6,
pp. 1197-1202,
06/01/1979
Copyright © 1979 by The American Society of Hematology
