|
|||||||||
|
|
|
|
|
|
|
|
|
|
|
MA Moore, AP Sheridan, TD Allen and TM Dexter
Maintenance of myelopoiesis and pluripotential stem cell production for
prolonged periods in vitro hitherto has been limited to mouse bone marrow
culture. In an effort to adapt the system for use in higher species,
particularly in human and non-human primates, studies were undertaken using
the prosimian species, Tupaia glis (tree shrew). In a number of experiments
the duration of sustained normal hematopoiesis observed in cultures of this
species, following a single inoculum of 5 X 10(6)--10(7) bone marrow cells,
with or without addition of fresh allogeneic bone marrow exceeded 1 yr.
Analysis of suspension cells obtained by weekly demidepopulation of such
cultures revealed production of CFU-C, differentiating neutrophils, and
basophils at high levels. Direct comparison with murine cultures indicated
that in both species a complex series of cellular interactions takes place
within an adherent environment of marrow-derived endothelial cells,
macrophages, and fat-containing cells. Certain functional and
ultrastructural features served to distinguish murine from Tupaia marrow
cultures, and the prolonged duration of in vitro hematopoiesis in the
latter species could be attributed to a regenerative capacity possessed by
its adherent hematopoietic microenvironment. The availability of this
primate marrow culture system should facilitate studies of hematopoiesis,
viral leukemogenesis, and transplantation biology, which have more direct
relevance to man than that provided by the existing murine system.
This article has been cited by other articles:
| ||||||||||
 |
 |
 |
|||||||
 |
 |
 |
 |
 |
 |
 |
 |
||
| Copyright © 1979 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
