Aggregation of hemoglobin S and hemoglobin CHarlem with nonsickle
hemoglobin in concentrated phosphate buffer
K Adachi, T Asakura and E Schwartz
The effect of HbA and HbF on both the aggregation and deaggregation of
deoxy HbS and deoxy HbCHarlem in concentrated phosphate buffers was studied
by a turbidimetric method. Although pure deoxy HbA is fully soluble in 2.4
M potassium phosphate buffer, the same concentration of deoxy HbAS was
insoluble in the same buffer. Since there was little dissolved hemoglobin
in the solute of a mixture of HbA and HbS, and since the intensity of
turbidity was approximately twice that which would be expected from deoxy
HbS alone, deoxy HbA must have coaggregated with deoxy HbS. Similar studies
with mixtures of HbS and HbF showed different results. The rates of
aggregation of deoxygenated mixtures of HbS and HbF were much slower than
those of similar mixtures of HbS and HbA. Measurements of the absorption
spectrum of the solute after centrifugation and the electrophoresis of both
the aggregates and the solutes showed that a portion of the HbF was
coaggregated with HbS, while some of the HbS was still in solution. The
solubility of HbS and mixtures of sickle and nonsickle hemoglobins in 2.2 M
phosphate buffer increased in the order of HbS, HbACHarlem, HbAS,
HbCHarlem, HbSF, and HbFCHarlem.
Volume 55,
Issue 3,
pp. 494-500,
03/01/1980
Copyright © 1980 by The American Society of Hematology
