In vivo radiolabeling of platelet proteins: a new method with
identification of platelet factor XIII
DM Rider, RP McDonagh and J McDonagh
A method for radiolabeling platelets in vivo was developed in which 3H-
arginine was injected into the bone marrow of normal dogs. On the third day
after injection, a maximum of 6%--7% of the radioactivity had been
incorporated into the total platelet mass. This method of isotope
administration resulted in a 50--60-fold increase in maximum uptake of
radiolabel by platelets, as compared to values obtained by others using
intravenous injections of various radioactive compounds. Tritium- labeled
platelets were harvested from the animals and then were washed to remove
unbound 3H-arginine. On polyacrylamide gel electrophoresis 7 labeled
protein bands, with molecular weights ranging from 29,000to 132,000, were
obtained from the platelet-soluble fraction. One 3H- containing protein
with a molecular weight of 81,000 was identified immunologically and
enzymatically as platelet factor XIII.
Volume 55,
Issue 4,
pp. 559-563,
04/01/1980
Copyright © 1980 by The American Society of Hematology
