Continuous human bone marrow culture: Ia antigen characterization of
probable pluripotential stem cells
MA Moore, HE Broxmeyer, AP Sheridan, PA Meyers, N Jacobsen and RJ Winchester
The presence of Ia-like antigens on human CFU-C and BFU-e is confirmed and
a cell type that lacked immediate capacity for granulocytic colony
formation but generated CFU-c after brief incubation in simple suspension
culture is identified. This pre-CFU-c, and its immediate progeny, was
extremely sensitive to killing by anti-Ia serum with complement. In
contrast, anti-Ia serum plus complement treatment of human bone marrow,
while eliminating 93%-97% of all CFU-c and BFU-e, did not prevent the rapid
regeneration of these progenitor cells and their production for some weeks
under the conditions of continuous marrow culture. These studies suggest
that the human equivalent of the pluripotential stem cell can replicate for
some weeks in culture and generate committed progenitors, such as CFU-c and
BFU-e. Furthermore, it would appear that Ia-like antigen is absent on the
pluripotential stem cell, is rapidly gained as commitment to the various
progenitor cell types occur, and is subsequently lost as these latter
undergo differentiation within the marrow.
Volume 55,
Issue 4,
pp. 682-690,
04/01/1980
Copyright © 1980 by The American Society of Hematology
