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Factor VIII-related protein circulates in normal human plasma as high
molecular weight multimers
LW Hoyer and JR Shainoff
The size of human factor VIII-related protein in plasma has been determined
by sodium dodecyl sulfate (SDS) glyoxyl agarose electrophoresis. The
protein was immobilized after the electrophoresis by coupling it to the
modified agarose, and it was identified by autoradiography using purified
rabbit anti-factor VIII-related antigen (VIIR:Ag). A series of multimeric
forms was identified with Mr of 0.85- 12 x 10(6). The distribution of
VIIR:Ag multimers was the same in heparin and citrate anticoagulated
plasmas and in serum, and the pattern was the same after freezing as in
plasma kept at 37 degrees C from the time of venipuncture until the
electrophoresis was complete. These observations indicate that VIIR:Ag
circulates in normal plasma as a population of very large multimers and
that the size distribution is not an artifact induced by purification
methods, freezing, or calcium chelation.
Volume 55,
Issue 6,
pp. 1056-1059,
06/01/1980
Copyright © 1980 by The American Society of Hematology
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