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Granulocyte aggregometry: a sensitive technique for the detection of C5a
and complement activation
DE Hammerschmidt, TK Bowers, CJ Lammi-Keefe, HS Jacob and PR Craddock
We have previously shown that complement (C) activated plasma causes
granulocyte (PMN) aggregation in vitro and that C5a is responsible. The
C-induced aggregation of PMNs treated with cytochalasin-B (CB) is markedly
enhanced and irreversible, and the magnitude of the response is
proportional to the log (concentration of activated plasma), allowing use
of this technique to detect C5a and hence C-activation. To compare the
sensitivity of granulocyte aggregometry to that of more standard methods of
detecting C-activation, we produced graded C- activation in vitro by
treating fresh serum with varying amounts of zymosan. Aggregometry was the
most sensitive index of C-activation, detecting C-activation, produced by
0.02 mg zymosan/ml of serum--1/10 that required to produce C-activation
detectable by C3 immunoelectrophoresis (the next most sensitive technique).
Granulocyte aggregometry may also be used to detect in vivo C-activation.
We have found aggregating activity in plasmas from patients with systemic
lupus erythematosus, immune vasculitis, transfusion reactions, and other
conditions associated with in vivo C-activation, but not in the plasmas of
normal subjects.
Volume 55,
Issue 6,
pp. 898-902,
06/01/1980
Copyright © 1980 by The American Society of Hematology
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