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T Olofsson and I Olsson
Low-density (less than 1.077 g/ml) marrow or blood cells from patients with
acute or chronic leukemia release a high molecular weight substance called
"leukemia-associated inhibitor" (LAI) that reduces the fraction of normal
marrow CFU-c in S-phase as measured with the 3H-TdR suicide technique. LAI
from conditioned media or 3M KCl extracts of subcellular fractions behaved
homogeneously on gel chromatography, showing an apparent molecular weight
greater than 500,000. However, ion- exchange chromatography and isoelectric
focusing indicated considerable charge heterogeneity for LAI molecules.
Results from SDS-polyacrylamide gel electrophoresis indicated that the
biologic activity resides in a subunit of 150,000-170,000 daltons. The
findings of marked affinity for Con-A-Sepharose, marked susceptibility to
mild periodate treatment, partial susceptibility to protease digestion, and
relative resistance to heating suggest that LAI is a glycoprotein. Data
from radiolabeling of cell surface components and sucrose density gradient
centrifugation are consistent with LAI being a peripheral cell membrane
glycoprotein, which may suppress normal granulopoiesis in leukemia.
This article has been cited by other articles:
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| Copyright © 1980 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
