Dose-dependent effects of a tumor promotor on blast cell progenitors in
human myeloblastic leukemia
LJ Chang and EA McCulloch
AML blast cell progenitors form colonies in culture when stimulated by a
media conditioned by leukocytes in th presence of PHA. Two cellular
processes occur during colony formation: self renewal generates new
progenitors, while others undergo a change that leads to decreased
proliferative potential. We tested the effect of the potent tumor promotor,
12-0-tetradecanoyl phorbol acetate (TPA) on these events. TPA was found to
be toxic to blast cell colony formation; doses in excess of 1 ng per ml
usually abolished it. At doses lower than this, self renewal, as determined
by replating either individual or pooled colonies, was increased. At
proliferation inhibiting TPA doses, surviving cells showed a spindle
morphology, and had increased ANA esterase activity. We interpret the data
to mean that TPA decreases blast cell maturation at low doses and may
increase it at high doses.
Volume 57,
Issue 2,
pp. 361-367,
02/01/1981
Copyright © 1981 by The American Society of Hematology
