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This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Hirano, M. Articles by Miyaji, T. Search for Related Content PubMed PubMed Citation Articles by Hirano, M. Articles by Miyaji, T. Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Hb Toyoake: beta 142 (H20) Ala replaced by Pro. A new unstable hemoglobin with high oxygen affinity M Hirano, Y Ohba, K Imai, T Ino, Y Morishita, T Matsui, S Shimizu, H Sumi, K Yamamoto and T Miyaji A new unstable hemoglobin with high oxygen affinity, Hb Toyoake: beta 142 (H20) Ala replaced by Pro, was found in Japanese male with a normal blood hemoglobin level, shortened red cell survival, and increased plasma erythropoietin. Hemoglobin studies showed heat and isopropanol instability, and an increased tendency to heme loss and to subunit dissociation. Electrophoresis of whole hemolysate showed inconstant abnormal bands with reduced mobilities due to progressive heme loss during the in vitro procedure. Isolated Hb Toyoake with normal heme content migrated slightly faster than HbA. Oxygen affinity of red cells was elevated with P50 of 17.0 mm Hg at pH 7.4 and 37 degrees C (normal 25.0 mm Hg). Studies on hemolysate implied that Hb Toyoake had an almost normal Bohr effect, a diminished cooperativity, and a reduced response to inositol hexaphosphate. These multiple abnormalities are associated with a substitution of Pro for beta 142 Ala, resulting in disruption of the H-helix and the adjacent C-terminal portion of beta chain, which contain residues crucial for normal oxygen binding. Volume 57, Issue 4, pp. 697-704, 04/01/1981 Copyright © 1981 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: P. Trifillis, K. Adachi, T. Yamaguchi, E. Schwartz, and S. Surrey Expression Studies of delta -Globin Gene Alleles Associated with Reduced Hemoglobin A2 Levels in Greek Cypriots J. Biol. Chem., October 25, 1996; 271(43): 26931 - 26938. [Abstract] [Full Text] [PDF]

	Copyright © 1981 by American Society of Hematology         Online ISSN: 1528-0020