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Purification of erythroblastic nests

AJ Macario, C Dugan, IL Perez-Lloret and E Conway de Macario

We describe a method for preparing purified erythroblastic nests in large numbers (approximately 10(6)/run) in three steps: (1) induction of splenic erythropoiesis in mice, (2) preparative differential centrifugation for the removal of erythrocytes and single cells from spleen cell suspensions, and (3) sedimentation in an isokinetic gradient of Ficoll 400 in Joklik's modification of minimum essential medium. Viability of isolated EN is very high, as demonstrated by the trypan blue exclusion and in vitro erythrocyte formation methods.

Volume 57, Issue 5, pp. 922-927, 05/01/1981
Copyright © 1981 by The American Society of Hematology


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  Copyright © 1981 by American Society of Hematology         Online ISSN: 1528-0020