Humoral helper activity for B-cell differentiation released from non-
Hodgkin's lymphoma cells having both SRBC and complement receptors in the
pokeweed mitogen system
N Moriya, T Miyawaki, Y Ueno, S Koizumi and N Taniguchi
The majority of lymphoid cells from a patient with non-Hodgkin's lymphoma
with leukemic transformation were demonstrated to carry receptors for both
sheep erythrocytes and complements by the combined rosette assay using
neuraminidase-treated sheep erythrocytes and complement-coated zymosan
beads. Most of them were considered morphologically lymphoblasts and were
positive for acid phosphatase staining. Terminal deoxynucleotidyl
transferase activity was not detected in these cells. Lymphoid cells from
this patient did not respond to the stimulation with phytohemagglutinin-P,
concanavalin-A, and pokeweed mitogen (PWM). When these cells were cultured
with PWM for 7 days, no plasma cell was generated. Although only a few
plasma cells were generated in the PWM-stimulated culture of normal
purified B cells alone, the addition of the patient's cells to purified
normal B cells resulted in a markedly enhanced generation of plasma cells
in response to PWM, as was the case with normal T cells. But leukemic cells
either from a patient with T-cell leukemia not having complement receptors
or from a patient with null-cell leukemia showed no enhancing ability in B-
cell differentiation. In addition, the culture supernates of the patient's
cells obtained after 24-hr PWM stimulation had an ability to promote B-cell
differentiation comparable in activity to those from the PWM-stimulated
normal T cells.
Volume 57,
Issue 6,
pp. 1074-1080,
06/01/1981
Copyright © 1981 by The American Society of Hematology
