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Eosinophil autofluorescence and its use in isolation and analysis of human
eosinophils using flow microfluorometry
GJ Weil and TM Chused
Unstained human eosinophils exhibit unusually bright autofluorescence,
which allows them to be distinguished from other leukocytes using
fluorescence microscopy. Eosinophil fluorescence is associated with the
cytoplasmic granules of the cells. Eosinophil granule extracts, containing
an as-yet-undefined eosinophil fluorescence factor, exhibited excitation
maxima at 370 nm and 450 nm, with maximum emission at 520 nm. Eosinophils
adhering to opsonized parasites in vitro deposit fluorescent material onto
the parasite surface. Eosinophil fluorescence was of sufficient intensity
to allow the preparation of viable, highly enriched (greater than or equal
to 98%), eosinophil suspensions from peripheral blood of normal and
eosinophilic donors using a fluorescence- activated cell sorter.
Quantitative studies of eosinophil autofluorescence were performed using
flow microfluorometry. Fluorescence intensity of blood eosinophils from
normal volunteers and eosinophilic patients varied inversely with the log
of the donor's absolute eosinophil count regardless of clinical diagnosis.
Volume 57,
Issue 6,
pp. 1099-1104,
06/01/1981
Copyright © 1981 by The American Society of Hematology
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