Abnormal phosphoenolpyruvate transport in erythrocytes of hereditary
spherocytosis
H Ideguchi, N Hamasaki and Y Ikehara
The rate of phosphoenolpyruvate transport in erythrocytes from patients
with hereditary spherocytosis and from healthy individuals was examined in
a 0.1 M citrate buffer (pH 6.1 at 37 degrees C) containing 10 mM
phosphoenolpyruvate and 10 mM NaF. The rate in erythrocytes from patients
with hereditary spherocytosis was 0.09 +/- 0.02 mumol/min/ml of cells (mean
+/- SD, n = 8), whereas the rate in erythrocytes from healthy individuals
was 0.23 +/k- 0.03 mumol/min/ml of cells (mean +/- SD, n = 8). The lower
rate of transport in erythrocytes of hereditary spherocytosis could not be
described to a different glycolytic response to NaF or to a difference in
cell age distribution. This phenomenon seemed to be a reflection of some
abnormality in the erythrocyte membrane from patients with hereditary
spherocytosis.
Volume 58,
Issue 3,
pp. 426-430,
09/01/1981
Copyright © 1981 by The American Society of Hematology