Effects of acetyl glyceryl ether phosphorylcholine on human platelet
function in vitro
AJ Marcus, LB Safier, HL Ullman, KT Wong, MJ Broekman, BB Weksler and KL Kaplan
AGEPC (PAF), at 1.9 x 10(-8) M or higher, induced concentration- dependent
aggregation and release in human platelet-rich plasma. Comparative studies
with arachidonate, collagen, ionophore, and ADP suggested that AGEPC was a
strong stimulus for platelet aggregation and probably a moderate agonist
for release, as well as a relatively weak inducer of TXA2 production. The
initial phase of AGEPC-induced aggregation was independent of ADP release
and TXA2 formation, since it was not inhibited by ASA, apyrase, or CP/CPK.
Whereas irreversible aggregation always required ADP release, TXA2
formation was not essential in each instance. Thus, in several experiments,
full aggregation responses took place in AGEPC-stimulated platelets that
had been pretreated with ASA. AGEPC-induced release of 5-HT, beta -
thromboglobulin and PF-4 occurred in parallel and were inhibited by both
apyrase and ASA. Washed human platelets did not respond to exogenous AGEPC
in the absence of ADP and did not appear to generate significant quantities
of AGEPC upon stimulation with thrombin or ionophore.
Volume 58,
Issue 5,
pp. 1027-1031,
11/01/1981
Copyright © 1981 by The American Society of Hematology
