Long-term suspension cultures of human cord blood myeloid cells
SZ Salahuddin, PD Markham, FW Ruscetti and RC Gallo
A method is described that allows for the routine long-term (greater than 3
mo) growth of normal, immature human myeloid cells in liquid suspension
culture. The techniques employ cell separation, utilization of special
growth conditions (RPMI with hydrocortisone and vitamin D), and cord blood
as the source of leukocytes. These cultures are composed predominantly of
immature myeloid cells that have grown for over 3 mo in culture but
eventually terminate as differentiated, mature granulocytes and
monocyte-macrophages. Application of these techniques to other sources of
fresh human leukocytes (bone marrow and adult peripheral blood) resulted in
only short-term proliferation of myeloid cells. The techniques described
can be used for the routine expansion of immature myeloid and monocytoid
cell populations, particularly from newborns, and should be useful for
systematic studies of normal myeloid- monocytoid cell growth and
differentiation and providing normal control cells in studies employing
human leukemic myeloid cells.
Volume 58,
Issue 5,
pp. 931-938,
11/01/1981
Copyright © 1981 by The American Society of Hematology
