Analysis of antigenic determinants on human monocytes and macrophages
RF Todd and SF Schlossman
Mo1, 2, 3, and 4, and Plt-1 are a series of five distinct antigens detected
on the surface of human peripheral blood monocytes by mouse monoclonal
antibodies. Mo2 and 3 are restricted to the monocyte- macrophage series,
while Mo1, as previously reported, is also expressed by human granulocytes
and null cells. Mo3, as distinguished from Mo1 and Mo2, is weakly expressed
by virgin peripheral blood monocytes but becomes well expressed if
monocytes are cultured overnight at 37 degrees C. Mo4 is coexpressed by
monocytes and platelets, while Plt-1 appears to be a platelet-specific
antigen whose detection on monocytes reflects adherence of platelets to
monocyte membranes. That Mo2-4 are true monocyte antigens is demonstrated
by their resynthesis following protease treatment of monocytes (Mol
expression is resistant to proteolytic digestion). During myeloid-monocyte
differentiation, the Mo antigens are infrequently expressed by immature
myeloid cells but are found at higher frequency on leukemic monocytic
forms. Macrophages from cultured peripheral blood monocytes and HL-60 cells
exposed to lymphokines or phorbol diester express Mo1-4, but noncirculating
peritoneal macrophages lack Mo3. The Mo antigens are differentiation
markers whose expression reflects membrane heterogeneity during myeloid-
monocyte-macrophage maturation.
Volume 59,
Issue 4,
pp. 775-786,
04/01/1982
Copyright © 1982 by The American Society of Hematology
