Two classes of murine granulocyte progenitor cells expressed in plasma clot
diffusion chamber cultures
HN Steinberg, PL Page and SH Robinson
Two distinct classes of granulocyte progenitor cells present in normal
mouse bone marrow are expressed sequentially in the vivo plasma clot
diffusion chamber culture system. By several criteria, progenitor cells
giving rise to granulocyte colonies on day 4 of culture (CFU-D4) are
different from those giving rise to colonies on day 7 (CFU-D7). These
differences include: cell cycle activity as measured by in vitro incubation
with cytosine arabinoside, residual concentration in the bone marrow after
in vivo treatment of donor mice with cytosine arabinoside or methotrexate,
resistance to osmotic lysis, size as determined by velocity sedimentation,
and the morphology of the granulocyte colonies to which these cells give
rise. The CFU-D7 appears to represent an earlier progenitor cell than the
CFU-D4 in the differentiation pathway of the granulocyte and is analagous
in many respects to the BFU-E in the erythroid pathway.
Volume 59,
Issue 4,
pp. 838-843,
04/01/1982
Copyright © 1982 by The American Society of Hematology
