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In vitro regulation of immunoglobulin synthesis after human marrow
transplantation. II. Deficient T and non-T lymphocyte function within 3- 4
months of allogeneic, syngeneic, or autologous marrow grafting for
hematologic malignancy
RP Witherspoon, LG Lum, R Storb and ED Thomas
Immunoglobulin secretion was studied in 37 patients between 19 and 106 days
after allogeneic HLA-identical (30 patients), allogeneic one HLA-
haplotype-identical (three patients), syngeneic (three patients), or
autologous (one patient) marrow grafting. E rosette-positive (T) and E
rosette-negative (non-T) peripheral blood mononuclear cells were cocultured
with pokeweed mitogen for 6 days. Polyvalent immunoglobulin secretion was
determined by counting plaque forming cells in a reverse hemolytic plaque
assay. The number of antibody secreting cells in cocultures of autologous T
and non-T lymphocytes was low in 40 of 44 tests conducted on samples from
the 37 patients. Mononuclear or non-T cells from 38 of 40 tests failed to
produce antibody when cultured with normal helper T cells. T cells from 23
of 37 tests failed to help normal non-T cells secrete antibody. T
lymphocytes from 23 of 41 tests suppressed antibody production greater than
80% by normal T and non-T cells. The suppressor cells were radiosensitive
in 17 of the 25 tests. The abnormal function of lymphocyte subpopulations
in patients during the first 3 mo after syngeneic, allogeneic or autologous
marrow grafting was similar regardless of the type of graft or the presence
of acute graft versus host disease.
Volume 59,
Issue 4,
pp. 844-850,
04/01/1982
Copyright © 1982 by The American Society of Hematology
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