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The immunologic phenotyping of bone marrow biopsies and aspirates: frozen
section techniques
GS Wood and RA Warnke
Techniques were developed for the preparation of frozen sections from
undecalcified bone marrow biopsy cores and bone marrow aspirates. Selected
indirect antibody and biotin-avidin detection systems, employing
immunoperoxidase or immunofluorescent labels, were studied to determine
which were best suited for bone marrow frozen section immunohistopathology.
Methods employing murine hybridoma monoclonal antibodies illustrate the
immunophenotyping of representative lymphoid neoplasms, involving bone
marrow. The results of immunohistologic staining were comparable to those
of fluorescence-activated cell sorter (FACS) analysis of cell suspensions.
The advantages and limitations of immunohistologic techniques are discussed
as they relate to immunophenotypic studies previously feasible only with
bone marrow cell suspensions. Frozen section immunohistologic techniques
serve as useful adjuncts to the conventional evaluation of bone marrow
aspirates and biopsies.
Volume 59,
Issue 5,
pp. 913-922,
05/01/1982
Copyright © 1982 by The American Society of Hematology
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