Macrophage differentiation of human precursor B-cell line by phorbol ester
and colony-stimulating factor
A Hiraoka, K Kubota, HD Preisler and J Minowada
A human round cell line, KLM-2, is considered to be precursor B-cell by
immunologic surface marker analysis and histochemical studies. The effect
of 12-O-tetradecanoyl phorbol-13-acetate (TPA) and/or colony- stimulating
factor (CSF) on KLM-2 cells was investigated. KLM-2 cells became adherent
to the bottom of the flask on day 2 after liquid culture with TPA, and the
number of macrophage-like adherent cells (mAC) with pseudopodia showed a
TPA dose-dependent increase to the peak level on day 3 or 4. The mAC had a
phagocytic activity. The suspending cells were positive for alpha-naphthyl
acetate esterase (NAE) up to about 50% in culture with TPA. A TPA
concentration of 100 ng/ml was optimal to provide a maximum number of mAC
and NAE-positive suspending cells. Particularly, such effects of TPA on
KLM-2 cells were markedly accelerated in the presence of CSF. These
findings indicate macrophage differentiation of KLM-2 cells in the presence
of TPA and CSF. KLM-2 cells may be a bipotential stem cell line
differentiating into B-cell and monocyte-macrophage lineages.
Volume 59,
Issue 5,
pp. 997-1000,
05/01/1982
Copyright © 1982 by The American Society of Hematology
