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Factors that affect human hemopoiesis are produced by T-cell growth factor
dependent and independent cultured T-cell leukemia-lymphoma cells
C Tarella, FW Ruscetti, BJ Poiesz, A Woods and RC Gallo
Some laboratory results and clinical situations suggest that human T cells
may be important in the regulation of growth of hematopoietic cells. Since
the discovery of T-cell growth factor (TCGF), systems are now available for
the long-term specific in vitro propagation of mature normal or neoplastic
human T cells, providing an opportunity to study the influence of T cells
on hematopoiesis. Recently, 24 cell lines from patients with cutaneous
T-cell lymphoma (CTCL) and T-cell acute lymphoblastic leukemia (T-ALL) were
grown with TCGF and then assessed for release of humoral factors that
affect hematopoiesis. Conditioned media (CM) from these cell lines were
tested for erythroid burst- promoting activity (BPA) and granulocyte
colony-stimulating activity (CSA). BPA was detected in CM from 3/6 cultures
of T-ALL patients and 4/6 CTCL cultures. CSA was found in the CM from 6/8
cultures of T-ALL patients, 7/12 CTCL cultures, and 3/4 CTCL cell lines
that become independent of exogenous TCGF for growth. The CSA from several
of the neoplastic T-cell cultures stimulated high levels of eosinophil
colonies, a possible source of the eosinophilia seen in these patients. The
ability of continuously proliferating human T lymphocytes, which retain
functional specificity and responsiveness to normal humoral regulation, to
produce factors that directly or indirectly stimulate myeloid and erythroid
colony formation lends further credence to the role of T lymphocytes in
regulating hematopoiesis.
Volume 59,
Issue 6,
pp. 1330-1336,
06/01/1982
Copyright © 1982 by The American Society of Hematology
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