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TJ Kunicki, MB Koenig, SM Kristopeit and RH Aster
An electroimmunoassay was applied to the quantitation of platelet-
associated IgG (PAIgG). Protein solubilized by Triton X100 from washed
platelets was electrophoresed at pH 5.0 in agarose gels containing
carbamoylated rabbit anti-human IgG (pI approximately equal to 5.0).
Because the rabbit antibody is immobilized under these conditions, while
PAIgG is freely mobile, rocket precipitates were produced, the heights of
which were directly proportional to the amount of antigen (PAIgG) present.
By this method, PAIgG for normal individuals was found to be 4.3 +/- 1.7
fg/platelet (mean +/- 2 SD; n = 35). Increased PAIgG levels (direct assay)
were found in 27 of 29 patients with a diagnosis of clinically active
idiopathic thrombocytopenic purpura (ITP), ranging from 10.5 to 101.5
fg/platelet. Moderately elevated PAIgG was found in 8 of 10 patients in an
early stage of recovery from ITP (range 7.5-9.5 fg/platelet) and in 3 of 6
patients with apparent nonimmune thrombocytopenia (range 14.5-24.0
fg/platelet). Electroimmunoassay for PAIgG can be performed on patients
with platelet counts as low as 2000/microliters, yields results in less
than 24 hr, is highly reproducible, and appears to provide a useful tool
for the evaluation of patients with immunologically mediated
thrombocytopenia.
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| Copyright © 1982 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
