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Clonal assay of mouse mast cell colonies in methylcellulose culture
T Nakahata, SS Spicer, JR Cantey and M Ogawa
When mouse marrow and spleen cells were cultured for over 12 days in
methylcellulose containing media conditioned by pokeweed-mitogen-
stimulated spleen cells, colonies containing mast cells and blast cells
were observed. The characteristic morphology of the colonies and the time
course of their development allowed in situ identification of the mast cell
colonies. Identification of the mast cells was confirmed by metachromatic
staining with toluidine blue and alcian blue, transmission electron
microscopy, and by demonstration of the membrane receptors for IgE.
Coculture studies with male and female marrow cells strongly indicated the
single cell origin of individual colonies. Detailed cytologic analyses of
mixed hemopoietic colonies and replating experiments of individual mixed
hemopoietic and mast cell colonies clearly established the hemopoietic
origin of mast cells. In replating experiments of individual mast cell
colonies, those without blast cells did not yield secondary mast cell
colonies. This result strongly indicated that morphologically recognizable
mast cells have lost their self-renewing capabilities. The quantitative
nature of the mast cell colony assay was supported by linearity studies and
provides a method for studies of the progenitors of mouse mast cells.
Volume 60,
Issue 2,
pp. 352-361,
08/01/1982
Copyright © 1982 by The American Society of Hematology
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|
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|
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|
|
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