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RT Perri, NE Kay, J McCarthy, RL Vessella, HS Jacob and LT Furcht
Control of neoplastic proliferation reflects in part monocyte/macrophage
destruction of target cells--destruction that evidently requires cell-cell
interaction. We herein show it to involve the natural plasma opsonin,
fibronectin. With two cultured human tumor lines--Malme melanoma and CAK-I
renal carcinoma cells--addition of fibronectin, purified to homogeneity,
enhances macrophage-mediated cytotoxicity 2--4-fold (p less than 0.01).
Both fresh human monocytes or the U-937-cultured macrophage line become
more lethal to tumor cells with added fibronectin. The fibronectin-enhanced
monocyte and U-937 tumoricidal activity occurred in a dose-dependent
fashion. Specificity of fibronectin's action was validated by use of
affinity-purified rabbit antifibronectin antibody, which completely abated
its enhancement of tumoricidal activity. Enhancement of tumoricidal
activity did not occur when monocytes or U-937 were exposed to
fibronectin-coated plates. However, the addition of soluble fibronectin to
fibronectin-coated plates was then capable of enhancing cytocidal activity.
These studies demonstrate that human fibronectin is capable of increasing
both fresh and cultured human monocyte tumor-directed cytotoxicity.
Fibronectin appears to be a potentially important circulating molecule that
may favorably influence human monocyte tumor cell cytotoxicity.
This article has been cited by other articles:
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| Copyright © 1982 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
