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Constitutive expression of platelet glycoproteins by the human leukemia cell line K562

AM Gewirtz, D Burger, TA Rado, EJ Benz and R Hoffman

The human leukemia cell line K562 was derived from a patient with chronic granulocytic leukemia. This cell line has subsequently been shown to possess phenotypic markers typical of erythroid and myeloid cells. Using a rabbit antiserum directed against purified platelet glycoproteins (PGPs), we have obtained evidence for the constitutive expression of PGPs on the surface of K562 cells. PGPs expressed have been tentatively identified as IIa and III based on their apparent migration in a 7% sodium dodecylsulfate polyacrylamide gel. K562 may become an important tool for the study of early events involved in megakaryocytic differentiation.

Volume 60, Issue 3, pp. 785-789, 09/01/1982
Copyright © 1982 by The American Society of Hematology


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