Generation of CFU-C suppressor T cells in vitro. III. Failure of
mitogen-primed T cells from patients with chronic granulocytic leukemia to
inhibit the growth of normal CFU-C
F Frassoni, A Bacigalupo, M Podesta, MT Van Lint, G Piaggio and A Marmont
T lymphocytes were derived by E rosetting from the peripheral blood (PB)
and bone marrow (BM) of 15 patients with chronic granulocytic leukemia
(CGL) in the chronic phase of their disease. T cells were also obtained
from 12 healthy individuals. T cells were incubated overnight either in
culture medium (RPMI) or RPMI plus pokeweed mitogen (PWM). The supernatants
were then recovered and the cells washed in fresh RPMI. T cells from normal
donors and from CGL patients were then cocultured with normal allogeneic
marrow cells grown in soft agar for CFU-C colony formation. Target marrow
cells were also grown in agar in the presence of T-derived supernatants.
The results of this study can be summarized as follows. (1) Normal PB and
BM T cells efficiently suppressed autologous and allogeneic CFU-C growth
after PWM stimulation. (2) T cells derived from peripheral blood or marrow
of CGL patients failed to inhibit CFU-C growth, whether pretreated with PWM
or not. (3) The supernatants of PWM-treated normal T cells strongly
inhibited CFU-C colony formation, whereas the supernatants of PWM- treated
CGL T cells had no CFU-C/suppressor activity. These data indicate that T
cells from CGL patients cannot be primed to become CFU- C suppressor cells
after PWM: stimulation in vitro and cannot release a soluble inhibitor of
granulopoiesis produced by PWM-primed normal T cells.
Volume 60,
Issue 6,
pp. 1447-1452,
12/01/1982
Copyright © 1982 by The American Society of Hematology
