Characterization of non-human primate antisera to acute lymphoblastic
leukemia (ALL): evidence for unique antigen(s) on childhood ALL of "T"
phenotype
T Mohanakumar, TW Coffey, MP Vaughn, EC Russell and D Conrad
A non-human primate antiserum was prepared to acute lymphoblastic leukemia
of T-cell phenotype (T-ALL) and, after absorptions with normal blood
elements, reacted by immunofluorescence and microcytotoxicity to all the
T-ALL tested. In addition, the antiserum reacted with cells from about 70%
of the common ALL studied and immunoprecipitated the common ALL antigen of
100,000 daltons. However, when the anti-T-ALL serum was absorbed with with
lymphoblasts from common ALL, it failed to react with common ALL
lymphoblasts, yet reacted significantly with cells from patients with T-ALL
phenotype and defined a 100,000-dalton membrane component not found on
common ALL lymphoblasts. In addition, sequential immunoprecipitation of
125I-labeled T-ALL membranes by anti- common-ALL serum followed by
anti-T-ALL serum detected the T-ALL membrane component of 100,000 daltons
that was not found on common ALL. Thus, our results demonstrate the
presence of of a unique human T-ALL antigen present on all T-ALL distinct
from the common ALL antigen.
Volume 61,
Issue 1,
pp. 66-70,
01/01/1983
Copyright © 1983 by The American Society of Hematology
