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Y Maede, M Inaba and N Taniguchi
We have found convincing evidence for the presence of Na-K-ATPase and high
potassium (K) and low sodium (Na) concentrations in the erythrocytes of
some dogs associated with hereditary high concentrations of erythrocyte
glutathione and some amino acids, glutamate, glutamine, and aspartate. The
Na-K-ATPase activity of the erythrocyte membranes of the dogs was about 3
times that of human erythrocyte membranes, whereas the enzyme activity was
not detected in control dogs with a normal level of blood glutathione. The
Michaelis constant of the enzyme for ATP (Km ATP) was 6.6 X 10(-3)M in the
dogs' erythrocytes and 5.0 X 10(-4)M in the human erythrocytes. The
concentration of K in the erythrocytes in the dogs examined was about 11
times that of the controls, whereas the erythrocyte Na concentration in the
dogs was about one-third that of the controls. The concentrations of K and
Na in the plasma of the dogs were equal to those of the controls.
Furthermore, L-3H-glutamate and L-3H-aspartate uptake by those cells with
high activity of Na-K-ATPase greatly increased, while L-3H-glutamine uptake
was unchanged. It appeared that Na+ and K+ gradients created by Na-K-ATPase
across the cell membrane might stimulate glutamate and aspartate uptake by
the cells, thus causing the high accumulation of such amino acids in the
cells.
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| Copyright © 1983 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
