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CF Whitfield, LM Mylin and SR Goodman
Two mammalian species (porcine and murine) have erythrocytes that are being
widely used to study membrane protein synthesis and red cell aging.
Erythrocytes of these species however, are significantly smaller than those
of the human. Before results obtained from study of these red cells can be
applied to human cells, the membrane skeleton of these species must be
investigated to determine if the skeletal elements are equivalent. Both pig
and mouse bands 4.1b were of lower molecular weight than human 4.1b, and
the a/b ratio was lower. In each species, 4.1a and b were sequence-related
phosphoproteins, and yielded substantially different one-dimensional
peptide maps. Band 3 of pig and mouse erythrocytes had a higher molecular
weight than human band 3 and also had differing one-dimensional peptide
maps after limited proteolytic cleavage with three different enzymes. In
each species, free band 3 and band 3 bound to the membrane skeleton had
identical peptide maps. Other major membrane skeletal components (spectrin,
actin, and bands 2.1 and 4.2) seem to be very similar in molecular weight
in various species. These results demonstrate that the molecular weights
and relative proportions of the membrane skeletal elements are species
dependent.
This article has been cited by other articles:
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| Copyright © 1983 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
