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Monoclonal antibodies specific for globin chains
G Stamatoyannopoulos, M Farquhar, D Lindsley, M Brice, T Papayannopoulou and PE Nute
Six monoclonal antibodies specific for human globin chains are described.
They are produced by stable clones obtained by raising hybridomas using
cells of mice immunized with either adult or fetal hemoglobin.
Characterization of the antibodies included testing against tetrameric
human and other animal hemoglobins, isolated hemoglobin chains, and when
indicated, cyanogen bromide fragments. Monoclonals 16- 2 and 37-8 are
beta-chain specific. Antibody 31-2 recognizes an antigenic determinant
common to the alpha and beta subunits. Monoclonal 30-3 recognizes
determinants best expressed in the alpha 2 beta 2 tetramer. Antibody 45-1
recognizes a determinant common to beta and gamma subunits, while antibody
51-7 is gamma-chain specific. None of the monoclonal antibodies recognizes
mouse hemoglobin, and they display significant differences in binding to
hemoglobins of various species. The species-specific reactions and the
knowledge of the primary structures of globins allowed deductions about the
antigenic sites recognized by two of the monoclonals (16-2 and 45-1). These
antihemoglobin monoclonal antibodies will provide useful probes for
studying hemoglobin expression in vivo and in vitro.
Volume 61,
Issue 3,
pp. 530-539,
03/01/1983
Copyright © 1983 by The American Society of Hematology
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