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Extracellular matrix production by the adherent cells of long-term murine
bone marrow cultures
KS Zuckerman and MS Wicha
We have studied the deposition of extracellular matrix proteins in the
adherent stroma of long-term murine bone marrow cultures. Stable
hematopoiesis was maintained for greater than 12 wk. At selected intervals,
culture dishes were sacrificed by removing all nonadherent cells and air
drying the dishes. The adherent stromal layer was analyzed for the presence
of intracellular and extracellular collagen, fibronectin, and laminin using
double immunofluorescent staining with specific antisera against these
matrix components. In cultures examined during the first 2 wk, large
numbers of stromal cells contained collagen, fibronectin, and laminin. Over
the next 2 wk, an extensive extracellular network of fibronectin, laminin,
and collagen was deposited on the dishes, which persisted throughout the
life of the cultures. In contrast to a previous report, we detected
substantial numbers of endothelial cells by means of immunofluorescent
staining of stromal cells with antisera to type IV collagen, laminin, and
factor VIII antigen. Although deposition of these extracellular matrix
proteins coincides with onset of active hematopoietic cell production, the
relative roles of the stromal cells and the extracellular matrix in
supporting hematopoiesis in murine bone marrow cell cultures remain to be
determined.
Volume 61,
Issue 3,
pp. 540-547,
03/01/1983
Copyright © 1983 by The American Society of Hematology
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