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R Taetle, D To, A Caviles, SW Norby and J Mendelsohn
We performed a series of studies to further clarify the nature of
lymphocyte colony-forming cells (CFC) from normal peripheral blood.
Mononuclear cells were separated into E-rosette-enriched (E+) and E-
rosette-depleted (E-) populations and cultured in methylcellulose with
conditioned media and irradiated mononuclear cells. Linear plating
relationships were obtained with plating efficiencies of 0.26% +/- .02%
(mean +/- SE) for E+ CFC and 0.18% +/- .02% for E- CFC. Cells in E+
colonies were T lymphocytes and in E- colonies were B lymphocytes as
determined by cell surface marker analysis. Using the thymidine suicide
technique, approximately one-half of CFC were found to be in cycle at any
moment, and plating efficiencies and cell cycle status of E+ CFC were not
changed by preincubation with PHA in liquid culture for 48 hr. Using
antibody complement-mediated cytotoxicity, E+ CFC were found to be T101+,
OKT3+, and Ia-, while E- CFC were OKT3- and Ia+. Using monocyte-depleted
populations obtained by sedimentation at unit gravity, lymphocyte colony
growth was absent in monocyte-depleted fractions, and optimal growth
occurred with 40% monocytes in culture. In contrast to some previous
studies, we find that lymphocyte CFC originate from a small, cycling
population of cells bearing mature T or B lymphocyte markers. Entry into
cell division, however, does not confer colony-forming capacity on
lymphocytes. Monocytes are critical to growth of E+ CFC, and cultures
severely depleted of monocytes would not be expected to form colonies.
This article has been cited by other articles:
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| Copyright © 1983 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
