Characterization of human megakaryocyte colony-stimulating factor in the
urinary extracts from patients with aplastic anemia and idiopathic
thrombocytopenic purpura
M Kawakita, M Ogawa, E Goldwasser and T Miyake
Using procedures that were effective in the purification of human urinary
erythropoietin (Epo), we attempted initial purification of megakaryocyte
colony-stimulating factors (CSF) in urinary extracts from patients with
aplastic anemia (AA) and idiopathic thrombocytopenic purpura (ITP).
Comparison of colony stimulation by purified human Epo and crude urinary
extracts revealed: (1) that the pure Epo augments megakaryocyte colony
formation in culture and (2) MEG-CSF activity is also present in materials
other than Epo in the crude urinary extracts from the two types of
patients. Similar to purification of Epo, ethanol precipitation and
sulfopropyl-Sephadex chromatography provided twofold and threefold
increases in the specific activity of MEG-CSF, respectively. In contrast to
Epo, however, significant inactivation of MEG-CSF activity was seen with
phenol treatment. The elution profile of MEG-CSF seen on hydroxylapatite
chromatography of urinary extracts was different from that of Epo. These
data provided a basis for initial steps for purification of MEG-CSF and
support the notion that MEG-CSF is distinct from Epo.
Volume 61,
Issue 3,
pp. 556-560,
03/01/1983
Copyright © 1983 by The American Society of Hematology
