Surface antigenic determinants on human pluripotent and unipotent
hematopoietic progenitor cells
MP Bodger, CA Izaguirre, HA Blacklock and AV Hoffbrand
RFB-1 is a monoclonal antibody previously shown to react with
granulocyte-monocyte progenitors (CFU-GM) and immature lymphoid cells in
human bone marrow. RFB-HLA-DR is a monoclonal antibody that reacts with
HLA-DR (la-like) antigens. The present study shows that the bone marrow
subset reactive with both RFB-1 and RFB-HLA-DR contains all the cells that
give rise to mixed hematopoietic colonies (derived from CFU- GEMM; a
pluripotent human progenitor cell) as well as to megakaryocytic
(megakaryocyte-CFU-derived) and erythropoietic (derived from erythroid
burst-forming units, BFU-E) colonies, as shown by fluorescence- activated
cell sorting and complement-mediated cytotoxicity. These results indicate
that CFU-GEMM, BFU-E, and megakaryocyte-CFU express RFB-1 and la-like
antigens. RFB-1 antigen is also expressed on erythroid colony-forming units
(CFU-E). RFB-1 and RFB-HLA-DR are useful reagents in the study of
hematopoietic stem cells.
Volume 61,
Issue 5,
pp. 1006-1010,
05/01/1983
Copyright © 1983 by The American Society of Hematology
