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Thirty-six human monoclonal immunoglobulins with antibody activity against
cytoskeleton proteins, thyroglobulin, and native DNA: immunologic studies
and clinical correlations
G Dighiero, B Guilbert, JP Fermand, P Lymberi, F Danon and S Avrameas
Six hundred and twelve monoclonal Ig (MIg) were studied for their antibody
activity against the following autoantigens: actin, tubulin, thyroglobulin,
myosin, myoglobin, fetuin, albumin, transferrin, and double-stranded DNA
(dsDNA). Of these 612 MIg, 36 (i.e., 5.75%) were shown to possess antibody
activity. Thirty-two of these 36 (5.22% of the total) were mainly directed
against actin. The four others were directed, respectively, against
tubulin, myosin, thyroglobulin, and dsDNA. The interaction of the MIg with
the respective antigen was demonstrated by immunoenzymatic methods with
monospecific antisera and by blotting experiments. Furthermore, this
interaction in the 12 cases studied was mediated by the dimeric fragment
F(ab')2 of the MIg. The MIg with antitubulin, antithyroglobulin, and
anti-dsDNA activities were exclusively inhibited by their homologous
antigens. Those with antiactin activity were predominantly inhibited by
actin and also by tubulin and thyroglobulin. The one binding to myosin was,
for the most part, inhibited by myosin and also significantly by actin and
tubulin. Retrospective clinical analysis was possible for 31/36 patients.
Twenty- six of 31 had malignant lymphoplasmocytic disorders. The five
others were followed for miscellaneous disorders without overt signs of
multiple myeloma (MM) or Waldenstrom's macroglobulinemia (WM). The
correlation between the antibody activity of the MIg and the clinical
features is discussed. These results indicate that a high proportion of MIg
possess antibody activity against actin (5.22%). This incidence contrasts
sharply with the positive reactions found toward all the other antigens
tested: only one each for dsDNA, tubulin, thyroglobulin, and myosin, and
none against myoglobin, fetuin, albumin, and transferrin. The significance
of these results and the relationship between MIg and natural antibodies
are discussed.
Volume 62,
Issue 2,
pp. 264-270,
08/01/1983
Copyright © 1983 by The American Society of Hematology
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