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Phagolysosomal pH of human neutrophils
P Cech and RI Lehrer
Human neutrophils sequester yeast cells or zymosan particles in two classes
of phagocytic vacuoles: sealed and unsealed. The pH of sealed vacuoles was
measured by a newly devised fluorometric procedure that used
fluoresceinated zymosan particles, derived from Saccharomyces cerevisiae,
as a pH probe. Five minutes after initiation of phagocytosis, sealed
vacuoles were alkaline, exhibiting a pH of 7.80 +/- 0.19 (mean +/- SEM).
Their acidity subsequently increased, so that the pH fell to 7.38 +/- 0.25
after 15 min. 6.35 +/- 0.35 after 30 min, and 5.68 +/- 0.26 after 60 min.
The implications of these findings for neutrophil microbicidal mechanisms
are discussed.
Volume 63,
Issue 1,
pp. 88-95,
01/01/1984
Copyright © 1984 by The American Society of Hematology
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