Monoclonal antibodies differentiating between monocytic and nonmonocytic
variants of AML
DC Linch, C Allen, PC Beverley, AG Bynoe, CS Scott and N Hogg
Blast cells from 70 cases of acute myeloid leukemia (AML), with a
preponderance of monocytic variants (M4 and M5), were stained with a panel
of 10 monoclonal antibodies (MoAb) that react with normal myeloid cells.
Two McAb, UCHMI and UCHALF, stained cells from nearly every case of M4 and
M5 leukemia, but not cells from cases of M1/M2 leukemia. Two other
antibodies, E11 and UC45, were also specific for the monocytic leukemias,
but stained cells from a lesser proportion of cases. The antibodies TG1,
OKM1, Smo, DA2, 28, and 5.5 stained a differing proportion of cases in both
the M1/M2 and M4/M5 subtypes. Routine morphology and alpha-naphthyl acetate
esterase (ANAE) staining were largely in agreement with the antibody
findings, though the interpretation of the fluoride sensitivity of ANAE was
apparently misleading in 10% of cases. It is concluded that these
antibodies, particularly UCHMI and UCHALF, will be of considerable value in
the more precise subtyping of AML and that some of this panel of antibodies
will be valuable tools for the investigation of normal monocytic
differentiation.
Volume 63,
Issue 3,
pp. 566-573,
03/01/1984
Copyright © 1984 by The American Society of Hematology
