Antigenic analysis of hematopoiesis: II. Expression of human neutrophil
antigens on normal and leukemic marrow cells
LC Strauss, KM Skubitz, JT August and CI Civin
The binding of five antineutrophil monoclonal antibodies AHN-1, -2, -3, -7,
and -8, to normal and leukemic bone marrow cells was studied. AHN-7 bound
to many granulocytic precursors, particularly myelocytes, and both lymphoid
and blast cells in normal marrow, and to most but not all
granulocyte-macrophage progenitors (CFC-GM). AHN-8 bound only to late (band
and segmented) neutrophilic cells and not to CFC-GM. AHN-1, -2, - 3 bound
to morphologically identifiable neutrophil precursors, but not to (day-14)
CFC-GM. Approximately half of nonlymphoid leukemia specimens were positive
with AHN-1 or AHN-7; by contrast, lymphoid leukemia specimens were rarely
positive. AHN-8 was rarely found on leukemic cells. These antineutrophil
antibodies appear to detect distinct granulopoietic subsets and may be
useful in the analysis of hematologic differentiation and in the
subclassification of leukemias.
Volume 63,
Issue 3,
pp. 574-578,
03/01/1984
Copyright © 1984 by The American Society of Hematology
