Increased tissue factor activity of monocytes/macrophages isolated from
canine renal allografts
H Rothberger, M Barringer and J Meredith
Kidney allografting was performed in a group of ten beagles, and viable
leukocytes infiltrating the transplanted organs were isolated during
episodes of acute rejection 5 or 6 days postoperatively. These infiltrate
populations, consisting predominantly of lymphocytes and
monocytes/macrophages, were found to have significantly increased amounts
of procoagulant activity relative to control leukocytes isolated from
circulating blood and lymph. Using nonspecific esterase staining in an agar
microclot assay, procoagulant activity in the infiltrate leukocytes was
found to reside in monocytes/macrophages rather than other coisolated cell
types. By contrast, control monocytes from blood had no activity in this
microclot assay. Procoagulant activity in the infiltrate cells was
characterized as tissue factor. Increased amounts of this activator of the
extrinsic pathway, as found in infiltrate monocytes/macrophages, may
initiate clotting reactions and fibrin deposition within allografts.
Volume 63,
Issue 3,
pp. 623-628,
03/01/1984
Copyright © 1984 by The American Society of Hematology
