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This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Griffin, J. D. Articles by Schlossman, S. F. Search for Related Content PubMed PubMed Citation Articles by Griffin, J. D. Articles by Schlossman, S. F. Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Induction of proliferation of purified human myeloid progenitor cells: a rapid assay for granulocyte colony-stimulating factors JD Griffin, R Sullivan, RP Beveridge, P Larcom and SF Schlossman The proliferation and differentiation of granulocyte and monocyte progenitor cells (CFU-C) in vitro is dependent on the presence of a group of closely related glycoproteins termed colony-stimulating factors (CSF). In order to investigate the interaction of these factors with CFU-C, we purified CFU-C from the peripheral blood of chronic myeloid leukemia patients with an immune rosette technique using specific monoclonal antibodies (mean 74-fold enrichment, 45% cloning efficiency). Colony formation by purified CFU-C demonstrated an absolute dependence on an exogenous source of CSF. Liquid culture of small aliquots of enriched CFU-C with CSF-containing medium resulted in a rapid, time- and concentration-dependent induction of DNA synthesis as measured by 3H-thymidine incorporation. This specific CSF induction of DNA synthesis by enriched CFU-C was used to develop a microassay system for CSF activity. CSF activity could be reproducibly quantitated in 24-48 hr. The proliferating cells in this assay system were shown to be myeloid progenitor cells by examining the morphology of their progeny and by determining the surface antigen phenotype of the responding cells (Ia+, T3-, B1-, Mo1-). This microassay provides a quantitative assessment of CSF activity that may be useful in the purification of human CSF and in the generation of monoclonal antibodies to CFU-C surface structures. Volume 63, Issue 4, pp. 904-911, 04/01/1984 Copyright © 1984 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: I. Virgolini, S.-R. Li, Q. Yang, E. Koller, W. R. Sperr, M. L. P. Angelberger, J. Nimpf, W. Schneider, and P. Valent Characterization of LDL and VLDL Binding Sites on Human Basophils and Mast Cells Arterioscler Thromb Vasc Biol, January 1, 1995; 15(1): 17 - 26. [Abstract] [Full Text]

	Copyright © 1984 by American Society of Hematology         Online ISSN: 1528-0020