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N Kamatani, H Yamanaka, K Nishioka, T Nakamura, K Nakano, K Tanimoto, T Mizuno and Y Nishida
Thioguanine-resistant T lymphoblast populations were selectively amplified
using T cell growth factor in the cultures of peripheral blood T cells from
four Lesch-Nyhan heterozygotes. Although Lesch-Nyhan T lymphoblasts were
all thioguanine-resistant, none of the cultures from 13 control subjects
yielded the growth of such defective cell populations. These data provide
direct evidence for the existence of a small percentage (5%-40%) of
hypoxanthine-guanine phosphoribosyltransferase (HGPRT) deficient T cells in
the heterozygotes, but not in normal individuals. Conversely, culture of
the T lymphoblasts with azaserine plus hypoxanthine permitted the growth of
the other part of the cell population that was enzyme positive. The low
percentages of HGPRT-negative cells among T cells in heterozygotes suggest
that the presence of this enzyme is beneficial for differentiation of
lymphocytes of T cell linkage. Considering the ease and the reliability,
culture of the peripheral T cells with thioguanine and T cell growth factor
is very likely of practical use for detecting Lesch-Nyhan syndrome carriers
among predisposed females.
This article has been cited by other articles:
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| Copyright © 1984 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
