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EA Machado, DA Gerard, CB Lozzio, BB Lozzio, JR Mitchell and DW Golde
To study the influence of a biologic environment on cultured human leukemia
cells, KG-1, KG-1a, and HL-60 cells were inoculated subcutaneously into
newborn nude mice. The cells developed myelosarcomas at the site of
inoculation and in lungs and kidneys. KG-1 and HL-60 myelosarcomas were
successfully passaged through adult nude mice, whereas KG-1a tumors
proliferated only after transplantation into newborn hosts. The human
nature of the cells forming myelosarcomas in mice was assessed by
chromosomal analyses and detection of cross- reactivity with an antibody to
the human leukemia cell line K562. We undertook electron microscopic and
cytochemical examinations of the cells proliferating in vitro and in the
mice. The granules of KG-1 cells in vivo did not react for acid
phosphatase, as observed in vitro, and the HL-60 cells proliferating in
mice lost the perinuclear myeloperoxidase (MPO) demonstrated in cultured
cells. Although the influence of an in vivo selection of cell
subpopulations cannot be ruled out, the enzymatic changes are compatible
with induced cell differentiation. Conclusive evidence of differentiation
in vivo was observed in the KG-1a cell subline. The undifferentiated KG-1a
blasts developed cytoplasmic granules and synthesized MPO during
proliferation in vivo. These observations indicate that human leukemia
cells from established cell lines proliferate in nude mice and may acquire
new differentiated properties in response to the in vivo environment.
This article has been cited by other articles:
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| Copyright © 1984 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
